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1

Native-State Heterogeneity of β2-Microglobulin as Revealed by Kinetic Folding and Real-Time NMR Experiments

Mukaiyama, Atsushi, Nakamura, Takashi, Makabe, Koki, Maki, Kosuke, Goto, Yuji, Kuwajima, Kunihiro

Journal:

Journal of Molecular Biology

Year:

2013

Language:

english

File:

PDF, 701 KB

english, 2013

2

Acid Denaturation and Refolding of Green Fluorescent Protein †

Enoki, Sawako, Saeki, Kimiko, Maki, Kosuke, Kuwajima, Kunihiro

Journal:

Year:

2004

Language:

english

File:

PDF, 276 KB

english, 2004

3

Application of the pH-jump method to the titration of tyrosine residues in bovine .alpha.-lactalbumin

Kuwajima, Kunihiro, Ogawa, Yuji, Sugai, Shintaro

Journal:

Year:

1979

Language:

english

File:

PDF, 581 KB

english, 1979

4

The Use of the Time-Resolved X-Ray Solution Scattering for Studies of Globular Proteins

Kuwajima, Kunihiro, Arai, Munehito, Inobe, Tomonao, Ito, Kazuki, Nakao, Masaharu, Maki, Kosuke, Kamagata, Kiyoto, Kihara, Hiroshi, Amemiya, Yoshiyuki

Journal:

Spectroscopy / An International Journal

Year:

2002

Language:

english

File:

PDF, 1.32 MB

english, 2002

5

Residual Structure of Unfolded Ubiquitin as Revealed by Hydrogen/Deuterium-Exchange 2D NMR

Yagi-Utsumi, Maho, Chandak, Mahesh S., Yanaka, Saeko, Hiranyakorn, Methanee, Nakamura, Takashi, Kato, Koichi, Kuwajima, Kunihiro

Journal:

Biophysical Journal

Year:

2020

File:

PDF, 6.90 MB

2020

6

Hydrogen exchange study of canine milk lysozyme: Stabilization mechanism of the molten globule

Yoshihiro Kobashigawa, Makoto Demura, Takumi Koshiba, Yasuhiro Kumaki, Kunihiro Kuwajima, Katsutoshi Nitta

Journal:

Proteins: Structure, Function, and Bioinformatics

Year:

2000

Language:

english

File:

PDF, 310 KB

english, 2000

7

Folding-unfolding of goat α-lactalbumin studied by stopped-flow circular dichroism and molecular dynamics simulations

Takao Yoda, Minoru Saito, Munehito Arai, Katsunori Horii, Kouhei Tsumoto, Masaaki Matsushima, Izumi Kumagai, Kunihiro Kuwajima

Journal:

Proteins: Structure, Function, and Bioinformatics

Year:

2001

Language:

english

File:

PDF, 528 KB

english, 2001

8

Mutational analysis of protein solubility enhancement using short peptide tags

Atsushi Kato, Kosuke Maki, Teppei Ebina, Kunihiro Kuwajima, Kunitsugu Soda, Yutaka Kuroda

Journal:

Year:

2007

Language:

english

File:

PDF, 268 KB

english, 2007

9

Strategy for trapping intermediates in the folding of ribonuclease and for using 1H-NMR to determine their structures

Kunihiro Kuwajima, Peter S. Kim, Robert L. Baldwin

Journal:

Year:

1983

Language:

english

File:

PDF, 567 KB

english, 1983

10

Hydrogen exchange of the tryptophan residues in bovine α-lactalbumin studied by uv spectroscopy

Yoshiaki Harushima, Kunihiro Kuwajima, Shintaro Sugai

Journal:

Year:

1988

Language:

english

File:

PDF, 1005 KB

english, 1988

11

Equilibrium and kinetics of the thermal unfolding of α-lactalbumin. The relation to its folding mechanism

Kunihiro Kuwajima, Shintaro Sugai

Journal:

Biophysical Chemistry

Year:

1978

Language:

english

File:

PDF, 809 KB

english, 1978

12

Calcium-regulated interactions of human α-lactalbumin with bee venom melittin

Eugene A. Permyakov, Valery M. Grishchenko, Lina P. Kalinichenko, Nikolay Y. Orlov, Kunihiro Kuwajima, Shintaro Sugai

Journal:

Biophysical Chemistry

Year:

1991

Language:

english

File:

PDF, 551 KB

english, 1991

13

Solution X-ray scattering study on the chaperonin GroEL from Escherichia coli

Yoshihiko Igarashi, Kazumoto Kimura, Kaoru Ichimura, Shigeru Matsuzaki, Teikichi Ikura, Kunihiro Kuwajima, Hiroshi Kihara

Journal:

Biophysical Chemistry

Year:

1995

Language:

english

File:

PDF, 743 KB

english, 1995

14

Protein folding in vitro

Kunihiro Kuwajima

Journal:

Current Opinion in Biotechnology

Year:

1992

Language:

english

File:

PDF, 732 KB

english, 1992

15

Structural characterization of the molten globule of α-lactalbumin by solution X-ray scattering

Mikio Kataoka, Fumio Tokunaga, Kunihiro Kuwajima, Yuji Goto

Journal:

Protein Science

Year:

1997

Language:

english

File:

PDF, 1.87 MB

english, 1997

16

Transition state in the folding of α-lactalbumin probed by the 6-120 disulfide bond

Masamichi Ikeguchi, Masao Kato, Shintaro Sugai, Motoaki Fujino, Kunihiro Kuwajima

Journal:

Protein Science

Year:

1998

Language:

english

File:

PDF, 2.08 MB

english, 1998

17

Contribution of Thr29 to the thermodynamic stability of goat α-lactalbumin as determined by experimental and theoretical approaches

Katsunori Horii, Minoru Saito, Takao Yoda, Kouhei Tsumoto, Masaaki Matsushima, Kunihiro Kuwajima, Izumi Kumagai

Journal:

Proteins: Structure, Function, and Bioinformatics

Year:

2001

Language:

english

File:

PDF, 407 KB

english, 2001

18

Non-native α-helix formation is not necessary for folding of lipocalin: Comparison of burst-phase folding between tear lipocalin and β-lactoglobulin

Seiichi Tsukamoto, Takako Yamashita, Yoshiteru Yamada, Kazuo Fujiwara, Kosuke Maki, Kunihiro Kuwajima, Yoshitaka Matsumura, Hiroshi Kihara, Hideaki Tsuge, Masamichi Ikeguchi

Journal:

Proteins: Structure, Function, and Bioinformatics

Year:

2009

Language:

english

File:

PDF, 296 KB

english, 2009

19

Correction to the article “Non-native α-helix formation is not necessary for folding of lipocalin: Comparison of burst-phase folding between tear lipocalin and β-lactoglobulin”

Seiichi Tsukamoto, Takako Yamashita, Yoshiteru Yamada, Kazuo Fujiwara, Kosuke Maki, Kunihiro Kuwajima, Yoshitaka Matsumura, Hiroshi Kihara, Hideaki Tsuge, Masamichi Ikeguchi

Journal:

Proteins: Structure, Function, and Bioinformatics

Year:

2009

Language:

english

File:

PDF, 34 KB

english, 2009

20

The molten globule state as a clue for understanding the folding and cooperativity of globular-protein structure

Kunihiro Kuwajima

Journal:

Proteins: Structure, Function, and Bioinformatics

Year:

1989

Language:

english

File:

PDF, 1.82 MB

english, 1989

21

Kinetic folding and unfolding of staphylococcal nuclease and its six mutants studied by stopped-flow circular dichroism

Nikolay N. Kalnin, Dr. Kunihiro Kuwajima

Journal:

Proteins: Structure, Function, and Bioinformatics

Year:

1995

Language:

english

File:

PDF, 1.28 MB

english, 1995

22

The allosteric transition of the chaperonin groel fromescherichia colias studied by solution X-ray scattering

Kunihiro Kuwajima, Tomonao Inobe, Munehito Arai

Journal:

Macromolecular Research

Year:

2006

Language:

english

File:

PDF, 375 KB

english, 2006

23

In reply —

Okazaki, Akira, Ikura, Teikichi, Kuwajima, Kunihiro

Journal:

Natural Structural Biology

Year:

1995

File:

PDF, 222 KB

1995

24

The chaperonin GroEL does not recognize apo-α-lactalbumin in the molten globule state

Okazaki, Akira, Ikura, Teikichi, Nikaido, Kiyokazu, Kuwajima, Kunihiro

Journal:

Natural Structural Biology

Year:

1994

File:

PDF, 643 KB

1994

25

Denaturation and reassembly of chaperonin GroEL studied by solution X-ray scattering

Munehito Arai, Tomonao Inobe, Kosuke Maki, Teikichi Ikura, Hiroshi Kihara, Yoshiyuki Amemiya, Kunihiro Kuwajima

Journal:

Protein Science

Year:

2003

Language:

english

File:

PDF, 213 KB

english, 2003

26

Sequential four-state folding/unfolding of goat α-lactalbumin and its N-terminal variants

Katsuaki Tomoyori, Takashi Nakamura, Koki Makabe, Kosuke Maki, Kimiko Saeki, Kunihiro Kuwajima

Journal:

Proteins: Structure, Function, and Bioinformatics

Year:

2012

Language:

english

File:

PDF, 544 KB

english, 2012

27

Thermodynamic characterization of partially denatured states in the denaturation process of bovine α-lactalbumin by inorganic denaturants

Satoshi Maruyama, Kunihiro Kuwajima, Katsutoshi Nitta, Shintaro Sugai

Journal:

Biochimica et Biophysica Acta (BBA) - Protein Structure

Year:

1977

Language:

english

File:

PDF, 569 KB

english, 1977

28

Intramolecular perturbation of tryptophans induced by the protonation of ionizable groups in goat α-lactalbumin

Kunihiro Kuwajima, Katsutoshi Nitta, Shintaro Sugai

Journal:

Biochimica et Biophysica Acta (BBA) - Protein Structure

Year:

1980

Language:

english

File:

PDF, 705 KB

english, 1980

29

Kinetic correlations between the disulfide bond reduction and the induced conformational change of proteins

Tatsuhisa Segawa, Kunihiro Kuwajima, Shintaro Sugai

Journal:

Biochimica et Biophysica Acta (BBA) - Protein Structure

Year:

1981

Language:

english

File:

PDF, 558 KB

english, 1981

30

Fibrillogenic propensity of the GroEL apical domain: A Janus-faced minichaperone

Jin Chen, Hisashi Yagi, Pietro Sormanni, Michele Vendruscolo, Koki Makabe, Takashi Nakamura, Yuji Goto, Kunihiro Kuwajima

Journal:

Year:

2012

Language:

english

File:

PDF, 1.45 MB

english, 2012

31

Nucleotide binding to the chaperonin GroEL: non-cooperative binding of ATP analogs and ADP, and cooperative effect of ATP

Tomonao Inobe, Tadashi Makio, Etsuko Takasu-Ishikawa, Tomoki P. Terada, Kunihiro Kuwajima

Journal:

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology

Year:

2001

Language:

english

File:

PDF, 388 KB

english, 2001

32

Thermodynamics of nucleotide binding to the chaperonin GroEL studied by isothermal titration calorimetry: evidence for noncooperative nucleotide binding

Tomoki P. Terada, Kunihiro Kuwajima

Journal:

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology

Year:

1999

Language:

english

File:

PDF, 271 KB

english, 1999

33

Rapid formation of a molten globule intermediate in refolding of α-lactalbumin

Munehito Arai, Kunihiro Kuwajima

Journal:

Folding and Design

Year:

1996

Language:

english

File:

PDF, 286 KB

english, 1996

34

Influence of Ca2+ binding on the structure and stability of bovine α-lactalbumin studied by circular dichroism and nuclear magnetic resonance spectra

Kunihiro Kuwajima, Yoshiaki Harushima, Dr. Shintaro Sugai

Journal:

Chemical Biology & Drug Design

Year:

1986

Language:

english

File:

PDF, 732 KB

english, 1986

35

Hydrophilic Residues at the Apical Domain of GroEL Contribute to GroES Binding but Attenuate Polypeptide Binding

Fumihiro Motojima, Tadashi Makio, Katsuhiko Aoki, Yoshihide Makino, Kunihiro Kuwajima, Masasuke Yoshida

Journal:

Biochemical and Biophysical Research Communications

Year:

2000

Language:

english

File:

PDF, 251 KB

english, 2000

36

Effect of GroEL on the Re-folding Kinetics of α-Lactalbumin

Kumiko Katsumata, Akira Okazaki, Kunihiro Kuwajima

Journal:

Journal of Molecular Biology

Year:

1996

Language:

english

File:

PDF, 403 KB

english, 1996

37

Dominant Forces in the Recognition of a Transient Folding Intermediate of α-Lactalbumin by GroEL

Kumiko Katsumata, Akira Okazaki, Galina P. Tsurupa, Kunihiro Kuwajima

Journal:

Journal of Molecular Biology

Year:

1996

Language:

english

File:

PDF, 374 KB

english, 1996

38

The Burst-phase Intermediate in the Refolding of β-Lactoglobulin Studied by Stopped-flow Circular Dichroism and Absorption Spectroscopy

Kunihiro Kuwajima, Hidetoshi Yamaya, Shintaro Sugai

Journal:

Journal of Molecular Biology

Year:

1996

Language:

english

File:

PDF, 604 KB

english, 1996

39

Kinetic refolding of β-lactoglobulin. Studies by synchrotron X-ray scattering, and circular dichroism, absorption and fluorescence spectroscopy

Munehito Arai, Teikichi Ikura, Gennady V Semisotnov, Hiroshi Kihara, Yoshiyuki Amemiya, Kunihiro Kuwajima

Journal:

Journal of Molecular Biology

Year:

1998

Language:

english

File:

PDF, 590 KB

english, 1998

40

Refolding kinetics of staphylococcal nuclease and its mutants in the presence of the chaperonin GroEL

Galina P Tsurupa, Teikichi Ikura, Tadashi Makio, Kunihiro Kuwajima

Journal:

Journal of Molecular Biology

Year:

1998

Language:

english

File:

PDF, 362 KB

english, 1998

41

Equilibrium and kinetics of the folding of equine lysozyme studied by circular dichroism spectroscopy

Mineyuki Mizuguchi, Munehito Arai, Yue Ke, Katsutoshi Nitta, Kunihiro Kuwajima

Journal:

Journal of Molecular Biology

Year:

1998

Language:

english

File:

PDF, 357 KB

english, 1998

42

Chaperonin-affected refolding of α-lactalbumin: effects of nucleotides and the co-chaperonin GroES

Tadashi Makio, Munehito Arai, Kunihiro Kuwajima

Journal:

Journal of Molecular Biology

Year:

1999

Language:

english

File:

PDF, 248 KB

english, 1999

43

Is folding of β-lactoglobulin non-hierarchic? intermediate with native-like β-sheet and non-native α-helix

Vincent Forge, Masaru Hoshino, Kazuo Kuwata, Munehito Arai, Kunihiro Kuwajima, Carl A Batt, Yuji Goto

Journal:

Journal of Molecular Biology

Year:

2000

Language:

english

File:

PDF, 370 KB

english, 2000

44

Fast folding of Escherichia coli cyclophilin A: a hypothesis of a unique hydrophobic core with a phenylalanine cluster

Teikichi Ikura, Toshiya Hayano, Nobuhiro Takahashi, Kunihiro Kuwajima

Journal:

Journal of Molecular Biology

Year:

2000

Language:

english

File:

PDF, 370 KB

english, 2000

45

Nucleotide-induced Transition of GroEL from the High-affinity to the Low-affinity State for a Target Protein: Effects of ATP and ADP on the GroEL-affected Refolding of α-Lactalbumin

Tadashi Makio, Etsuko Takasu-Ishikawa, Kunihiro Kuwajima

Journal:

Journal of Molecular Biology

Year:

2001

Language:

english

File:

PDF, 227 KB

english, 2001

46

An early immunoreactive folding intermediate of the tryptophan synthase β2 subunit is a ‘molten globule’

Michel E. Goldberg, Gennady V. Semisotnov, Bertrand Friguet, Kunihiro Kuwajima, Oleg B. Ptitsyn, Shintaro Sugai

Journal:

Year:

1990

Language:

english

File:

PDF, 835 KB

english, 1990

47

The Pro117 to glycine mutation of staphylococcal nuclease simplifies the unfolding—folding kinetics

Kunihiro Kuwajima, Naoki Okayama, Kaori Yamamoto, Tsuyoshi Ishihara, Shintaro Sugai

Journal:

Year:

1991

Language:

english

File:

PDF, 479 KB

english, 1991

48

Secondary structure of globular proteins at the early and the final stages in protein folding

Kunihiro Kuwajima, Gennady V Semisotnov, Alexei V Finkelstein, Shintaro Sugai, Oleg B Ptitsyn

Journal:

Year:

1993

Language:

english

File:

PDF, 521 KB

english, 1993

49

Absence of the thermal transition in apo-α-lactalbumin in the molten globule state: A study by differential scanning microcalorimetry

Katsuhide Yutani, Kyoko Ogasahara, Kunihiro Kuwajima

Journal:

Journal of Molecular Biology

Year:

1992

Language:

english

File:

PDF, 362 KB

english, 1992

50

Solubilization and folding of a fully active recombinant Gaussia luciferase with native disulfide bonds by using a SEP-Tag

Tharangani Rathnayaka, Minako Tawa, Takashi Nakamura, Shihori Sohya, Kunihiro Kuwajima, Masafumi Yohda, Yutaka Kuroda

Journal:

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics

Year:

2011

Language:

english

File:

PDF, 424 KB

english, 2011